All renal biopsies are processed for light microscopy and immunofluorescence
study. Biopsies are also processed for electron microscopy on a case to case
basis. A variety of immunohistochemical markers are available
Serial sections are stained with haematoxylin and eosin, periodic
acid–Schiff (PAS), Jones methenamine silver and Masson trichrome on
all biopsies.
A panel of additional histochemical stains are available, including Congo
red, Perls Prussian blue, Gomoris and Zeil Neelson stain.
All biopsies are stained with fluorescein isothiocyanate (FITC)-
conjugated rabbit antihuman antibodies to immunoglobulin G(IgG),
immunoglobulin A(IgA), immunoglobulin (IgM), C3,C1q, kappa and
lambda light chains.
Renal allograft biopsies are stained for C4d by the IF method
Anti PLA2r antibody, a marker of primary membranous nephropathy.
Staining for PLA2r for cases of membranous nephropathy to help the
nephrologist in choosing the treatment strategy.
Viral markers for transplant biopsies (CMV, SV40 for BK virus,
adenovirus ).
The other IHC markers include myoglobin, haemoglobin, IgG4,
and serum amyloid A.
DNAJB9 , a new and highly sensitive and specific
histological marker of fibrillary glomerulonephritis
